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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
18/12/2020 |
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Actualizado : |
18/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
COPPOLA, N.; FREIRE, B.; UMPIÉRREZ, A.; CORDEIRO, N.F.; AVILA. P.; TRENCHI, G.; CASTRO, G.; CASAUX, M.L.; FRAGA, M.; ZUNINO, P.; BADO, I.; VIGNOLI, R. |
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Afiliación : |
NADIA COPPOLA, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; BIBIANA FREIRE, Unidad Académica Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.; ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; NICOLÁS F. CORDEIRO, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; PABLO AVILA, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; GUSTAVO TRENCHI, Veterinario de Libre Ejercicio, Montevideo, Uruguay.; GUSTAVO CASTRO, Unidad Académica Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; INÉS BADO, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Transferable Resistance to Highest Priority Critically Important Antibiotics for Human Health in Escherichia coli Strains Obtained From Livestock Feces in Uruguay. (Brief Research Report Article). |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Frontiers in Veterinary Science, 19 November 2020, Volume 7, Article number 588919. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2020.588919 |
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DOI : |
10.3389/fvets.2020.588919 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 29 July 2020/ Accepted: 19 October 2020/Published: 19 November 2020. Correspondence: Rafael Vignoli, rvignoli@higiene.edu.uy; Inés Bado, ibado@higiene.edu.uy |
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Contenido : |
Abstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in which the search for transferable resistance to highest priority critically important antibiotics for human health is carried out in chickens and pigs chains of production animals in Uruguay. MenosAbstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in wh... Presentar Todo |
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Palabras claves : |
CMY-2; CTX-M-8; E. COLI; ESBL; ESCHERICHIA COLI; MCR-1; PLATAFORMA SALUD ANIMAL; POULTRY; QNRB19; SWINE. |
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Thesagro : |
AVES DE CORRAL; CERDO. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03119nam a2200409 a 4500
001 1061590
005 2020-12-18
008 2020 bl uuuu u0uu1 u #d
024 7 $a10.3389/fvets.2020.588919$2DOI
100 1 $aCOPPOLA, N.
245 $aTransferable Resistance to Highest Priority Critically Important Antibiotics for Human Health in Escherichia coli Strains Obtained From Livestock Feces in Uruguay. (Brief Research Report Article).$h[electronic resource]
260 $aFrontiers in Veterinary Science, 19 November 2020, Volume 7, Article number 588919. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2020.588919$c2020
500 $aArticle history: Received: 29 July 2020/ Accepted: 19 October 2020/Published: 19 November 2020. Correspondence: Rafael Vignoli, rvignoli@higiene.edu.uy; Inés Bado, ibado@higiene.edu.uy
520 $aAbstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in which the search for transferable resistance to highest priority critically important antibiotics for human health is carried out in chickens and pigs chains of production animals in Uruguay.
650 $aAVES DE CORRAL
650 $aCERDO
653 $aCMY-2
653 $aCTX-M-8
653 $aE. COLI
653 $aESBL
653 $aESCHERICHIA COLI
653 $aMCR-1
653 $aPLATAFORMA SALUD ANIMAL
653 $aPOULTRY
653 $aQNRB19
653 $aSWINE
700 1 $aFREIRE, B.
700 1 $aUMPIÉRREZ, A.
700 1 $aCORDEIRO, N.F.
700 1 $aAVILA. P.
700 1 $aTRENCHI, G.
700 1 $aCASTRO, G.
700 1 $aCASAUX, M.L.
700 1 $aFRAGA, M.
700 1 $aZUNINO, P.
700 1 $aBADO, I.
700 1 $aVIGNOLI, R.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
23/07/2020 |
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Actualizado : |
23/07/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GUIDO, A.; QUIÑONES, A.; PEREIRA, A.L.; SILVA, E.R. DA |
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Afiliación : |
ANACLARA GUIDO BOLIOLI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; AMPARO QUIÑONES DELLEPIANE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA LAURA PEREIRA AMATO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELIANE REGINA DA SILVA, Universidade Federal do Río Grande do Sul, Instituto de Biociencias, Depto. de botanica. Porto Alegre, RS, Brazil. |
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Título : |
Are the invasive grasses Cynodon dactylon and Eragrostis plana more phytotoxic than a co-occurring native? [¿Las gramíneas invasoras Cynodon dactylon y Eragrostis plana son más fitotóxicas que una nativa coexistente?]. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Ecología Austral, Agosto 2020, v. 30, p. 295-303. |
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Idioma : |
Inglés |
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Notas : |
History article: Recibido: 12 de Diciembre de 2019//Aceptado: 27 de Abril de 2020. |
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Contenido : |
ABSTRACT. Allelopathy, the release of phytotoxins by plants, may be a key mechanism by which an invasive species displaces residents. However, methodological procedures are still questioned in the literature. We evaluated the phytotoxic effects of Cynodon dactylon and Eragrostis plana, the most invasive grasses of the Río de la Plata grasslands. Although allelopathy has been claimed as one of the possible mechanisms involved in the establishment and impact of these species, no empirical evidence has convincingly supported it. We performed a germination experiment to assess the effect of both invasive species on Eragrostis bahiensis, a native grass from the region. We used aqueous extracts from fresh and dry leaves of three donor species: two invasive (C. dactylon and E. plana) and one co-occurring native from the introduced range (Coelorachis selloana). This approach allows to relativize the invasive species effect from resident species, according to the novel weapon hypothesis. As a result, only aqueous extracts from dried leaves inhibited the germination and early growth of E. bahiensis. The magnitude of these effects varied between donor species, but there was no consensus to conclude both invasive species had greater effects. Cynodon dactylon caused the strongest inhibitory effect on E. bahiensis. However, the native C. selloana also presented a potent inhibitory effect, stronger than the well-known invasive E. plana. Thus, the role of allelopathy on E. plana invasion in the Río de la Plata grasslands should be questioned. Finally, we addressed some suggestions for improving experimental design for testing the novelty of phytotoxic effects in the introduced range. MenosABSTRACT. Allelopathy, the release of phytotoxins by plants, may be a key mechanism by which an invasive species displaces residents. However, methodological procedures are still questioned in the literature. We evaluated the phytotoxic effects of Cynodon dactylon and Eragrostis plana, the most invasive grasses of the Río de la Plata grasslands. Although allelopathy has been claimed as one of the possible mechanisms involved in the establishment and impact of these species, no empirical evidence has convincingly supported it. We performed a germination experiment to assess the effect of both invasive species on Eragrostis bahiensis, a native grass from the region. We used aqueous extracts from fresh and dry leaves of three donor species: two invasive (C. dactylon and E. plana) and one co-occurring native from the introduced range (Coelorachis selloana). This approach allows to relativize the invasive species effect from resident species, according to the novel weapon hypothesis. As a result, only aqueous extracts from dried leaves inhibited the germination and early growth of E. bahiensis. The magnitude of these effects varied between donor species, but there was no consensus to conclude both invasive species had greater effects. Cynodon dactylon caused the strongest inhibitory effect on E. bahiensis. However, the native C. selloana also presented a potent inhibitory effect, stronger than the well-known invasive E. plana. Thus, the role of allelopathy on E. plana invasion in... Presentar Todo |
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Palabras claves : |
BERMUDAGRASS; CAPIM ANNONI; FITOTOXINAS; GRAMILLA; INVASION; LOVEGRASS; PASTIZALES DEL RIO DE LA PLATA; PHYTOTOXINS; RIO DE LA PLATA GRASSLANDS. |
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Asunto categoría : |
H60 Malezas y escardas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14556/1/Guido-2020.pdf
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Marc : |
LEADER 02683naa a2200277 a 4500
001 1061234
005 2020-07-23
008 2020 bl uuuu u00u1 u #d
100 1 $aGUIDO, A.
245 $aAre the invasive grasses Cynodon dactylon and Eragrostis plana more phytotoxic than a co-occurring native? [¿Las gramíneas invasoras Cynodon dactylon y Eragrostis plana son más fitotóxicas que una nativa coexistente?].$h[electronic resource]
260 $c2020
500 $aHistory article: Recibido: 12 de Diciembre de 2019//Aceptado: 27 de Abril de 2020.
520 $aABSTRACT. Allelopathy, the release of phytotoxins by plants, may be a key mechanism by which an invasive species displaces residents. However, methodological procedures are still questioned in the literature. We evaluated the phytotoxic effects of Cynodon dactylon and Eragrostis plana, the most invasive grasses of the Río de la Plata grasslands. Although allelopathy has been claimed as one of the possible mechanisms involved in the establishment and impact of these species, no empirical evidence has convincingly supported it. We performed a germination experiment to assess the effect of both invasive species on Eragrostis bahiensis, a native grass from the region. We used aqueous extracts from fresh and dry leaves of three donor species: two invasive (C. dactylon and E. plana) and one co-occurring native from the introduced range (Coelorachis selloana). This approach allows to relativize the invasive species effect from resident species, according to the novel weapon hypothesis. As a result, only aqueous extracts from dried leaves inhibited the germination and early growth of E. bahiensis. The magnitude of these effects varied between donor species, but there was no consensus to conclude both invasive species had greater effects. Cynodon dactylon caused the strongest inhibitory effect on E. bahiensis. However, the native C. selloana also presented a potent inhibitory effect, stronger than the well-known invasive E. plana. Thus, the role of allelopathy on E. plana invasion in the Río de la Plata grasslands should be questioned. Finally, we addressed some suggestions for improving experimental design for testing the novelty of phytotoxic effects in the introduced range.
653 $aBERMUDAGRASS
653 $aCAPIM ANNONI
653 $aFITOTOXINAS
653 $aGRAMILLA
653 $aINVASION
653 $aLOVEGRASS
653 $aPASTIZALES DEL RIO DE LA PLATA
653 $aPHYTOTOXINS
653 $aRIO DE LA PLATA GRASSLANDS
700 1 $aQUIÑONES, A.
700 1 $aPEREIRA, A.L.
700 1 $aSILVA, E.R. DA
773 $tEcología Austral, Agosto 2020$gv. 30, p. 295-303.
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